Bioinformatics Analysis Identifies IL6ST as a Potential Tumor Suppressor Gene for Triple-Negative Breast Cancer

Bioinformatics Analysis Identifies IL6ST as a Potential Tumor Suppressor Gene for Triple-Negative Breast Cancer

Improved perception into the molecular mechanisms of triple-negative breast most cancers (TNBC) is required to foretell prognosis and develop a new therapeutic technique for focused genes. The intention of this examine was to establish genes considerably related to TNBC and additional analyze their prognostic significance. The Cancer Genome Atlas (TCGA) TNBC database and gene expression profiles of GSE76275 from Gene Expression Omnibus (GEO) have been used to discover differentially co-expressed genes in TNBC in contrast with these in regular tissues and non-TNBC breast most cancers tissues. Differential gene expression and weighted gene co-expression community analyses recognized 24 differentially co-expressed genes.

Functional annotation prompt that these genes have been primarily enriched in processes such as metabolism, membrane, and protein binding. The protein-protein interplay (PPI) community additional recognized ten hub genes, 5 of which (MAPT, CBS, SOX11, IL6ST, and MEX3A) have been confirmed to be differentially expressed in an unbiased dataset (GSE38959). Moreover, CBS and MEX3A expression was upregulated, whereas IL6ST expression was downregulated in TNBC tissues in comparison with that in different breast most cancers subtypes. Furthermore, decrease expression of IL6ST was related to worse total survival in sufferers with TNBC. Thus, IL6ST may play an essential function in TNBC development and will serve as a tumor suppressor gene for prognosis and remedy.

Genetic variety evaluation of Dermacentor nuttalli inside Inner Mongolia, China

Ticks (Arthropoda, Ixodida), after mosquitoes, are the second most prevalent vector of infectious ailments. They are accountable for spreading a multitude of pathogens and threatening the well being and welfare of animals and human beings. However, given the historical past of tick-borne pathogen infections within the Inner Mongolia Autonomous Region of China, surprisingly, neither the genetic variety nor the spatial distribution of haplotypes inside ticks has been studied.  We characterised the haplotype distribution of Dermacentor nuttalli in 4 major pastoral areas of the Inner Mongolia Autonomous Region, by sampling 109 people (recovered from sheep) in April-August 2019.
The 16S rRNA gene, cytochrome c oxidase subunit I (COI), and the inner transcribed spacer 2 area (ITS2) have been amplified and sequenced from extracted DNA. Sodium valproate (VPA) is a histone deacetylase (HDAC) inhibitor, extensively prescribed within the remedy of bipolar dysfunction, and but the exact modes of therapeutic motion for this drug usually are not absolutely understood. After publicity of the rat serotonergic cell line RN46A to VPA, RNA-sequencing (RNA-Seq) evaluation confirmed widespread modifications in gene expression. Analysis by 4 bioinformatic pipelines revealed as many as 230 genes have been considerably upregulated and 72 genes have been considerably downregulated.
 Twenty-six haplotypes have been recognized utilizing 16S rRNA sequences, 57 haplotypes have been recognized with COI sequences, and 75 haplotypes have been recognized with ITS2 sequences. Among the three genes, complete haplotype variety was better than 0.7, whereas complete nucleotide variety was better than 0.06. Neutrality checks revealed a considerably unfavorable Tajima’s D consequence, whereas Fu’s Fs was not considerably constructive. Fixation index values (FST) indicated that the diploma of genetic differentiation amongst some sampled populations was small, whereas for others it was average.
Analysis of molecular variance (AMOVA) revealed that the variation inside populations was better than that amongst populations. The mismatch evaluation of D. nuttalli exhibited double peaks.  The genetic variety of D. nuttalli populations in our area can seemingly adapt to completely different geographical environments, thereby resulting in genetic variety, and creating genetic differentiation amongst completely different populations. However, genetic differentiation is cryptic and doesn’t type a pedigree geographical construction.
Bioinformatics Analysis Identifies IL6ST as a Potential Tumor Suppressor Gene for Triple-Negative Breast Cancer

Complete genome sequencing of Bacillus sp. TK-2, evaluation of its chilly evolution adaptability

 

To date, a massive variety of Bacillus species from completely different sources have been recognized. However, there are few investigations on genome info and evolutionary insights of Bacillus species from chilly environments. Bacillus sp. TK-2, remoted from the soil of Changbai Mountain, is a gram-positive bacterium with chilly adaptation traits. In this examine, we current the annotated full genome sequence of Bacillus sp. TK-2. The genome comprised 5,286,177 bp with a GC content material of 35.88%, 5293 protein-encoding genes, 32 rRNA, and 77 tRNA. Numerous genes associated to chilly adaptation have been detected within the genome of Bacillus sp. TK-2, primarily involving in vitality provide, regulation of cell membrane fluidity, antioxidant, and molecular chaperones.

In addition, the pressure TK-2 labeled within the Bacillus teams was distributed on a terminal department with Bacillus cereus A1 by Blastn and phylogenetic evaluation in NCBI database. Complete genome sequences of the pressure TK-2 and Bacillus cereus A1 have been in contrast by the net software “Average Nucleotide Identity”, exhibiting that the common nucleotide id of those two strains was 98.26%. In parallel, A comparative evaluation of the genomes of each Bacillus sp. TK-2 and Bacillus cereus A1 was carried out. Through the evaluation of core and particular genes with cd-hit, it was discovered that the 2 strains had 5691 pan gene, 4524 core gene, and 1167 particular gene clusters. Among the 624 particular gene clusters of Bacillus sp.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody

K3171120-II 1200 cm2
EUR 246
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti chicken secondary antibody

K3171120-III 1200 cm2
EUR 246
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti goat secondary antibody

K3171120-IV 1200 cm2
EUR 246
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti mouse secondary antibody

K3171250-I 2500 cm2
EUR 310
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody

K3171250-II 2500 cm2
EUR 310
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti chicken secondary antibody

K3171250-III 2500 cm2
EUR 310
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti goat secondary antibody

K3171250-IV 2500 cm2
EUR 310
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Western Blot Kit

20-abx098123
  • EUR 230.00
  • EUR 286.00
  • 100 ml
  • 200 ml
  • Shipped within 5-10 working days.

Western Blot Enhancing Kit

K3172120 1200 cm2
EUR 143
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Western Blot Enhancing Kit

K3172250 2500 cm2
EUR 188
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

TMB Substrate, for Western Blot

F100029 1000 ml
EUR 827
  • Product category: Buffer solution
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

TMB Substrate, for Western Blot

F129-100 100 ml
EUR 249
  • Product category: Buffer solution
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur.

Interferon alpha Western Blot Antibody

20-abx137014
  • EUR 356.00
  • EUR 523.00
  • 0.5 mg
  • 1 mg
  • Shipped within 5-10 working days.

AgileBlotter? Automated Western Blot Processor

ACT-ABH-40
EUR 16939

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-10 20mL (sufficient reagents for 4000 cm2 of membrane)
EUR 167

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-25 50mL (sufficient reagents for 10000 cm2 of membrane)
EUR 314

Hypersensitive Western Blot Chemiluminescent Substrate

CR0001-5 10mL (sufficient reagents for 2000 cm2 of membrane)
EUR 116

PDCD4 Western Blot kit (AWBK09045)

AWBK09045 10 reactions
EUR 647
Description:
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ETS1 Western Blot kit (AWBK100604)

AWBK100604 10 reactions
EUR 647
Description:
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ERG Western Blot kit (AWBK100625)

AWBK100625 10 reactions
EUR 647
Description:
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GABRP Western Blot kit (AWBK13034)

AWBK13034 10 reactions
EUR 647
Description:
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HTR1A Western Blot kit (AWBK13041)

AWBK13041 10 reactions
EUR 647
Description:
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RAB14 Western Blot kit (AWBK13107)

AWBK13107 10 reactions
EUR 647
Description:
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TNRC4 Western Blot kit (AWBK31525)

AWBK31525 10 reactions
EUR 647
Description:
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ESRRG Western Blot kit (AWBK31655)

AWBK31655 10 reactions
EUR 647
Description:
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GLI2 Western Blot kit (AWBK31885)

AWBK31885 10 reactions
EUR 647
Description:
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PAX4 Western Blot kit (AWBK32064)

AWBK32064 10 reactions
EUR 647
Description:
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AHR Western Blot kit (AWBK32243)

AWBK32243 10 reactions
EUR 647
Description:
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GLI1 Western Blot kit (AWBK32368)

AWBK32368 10 reactions
EUR 647
Description:
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PAX7 Western Blot kit (AWBK32393)

AWBK32393 10 reactions
EUR 647
Description:
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LHX6 Western Blot kit (AWBK32553)

AWBK32553 10 reactions
EUR 647
Description:
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HIPK2 Western Blot kit (AWBK32586)

AWBK32586 10 reactions
EUR 647
Description:
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PAX7 Western Blot kit (AWBK32742)

AWBK32742 10 reactions
EUR 647
Description:
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ZFP67 Western Blot kit (AWBK32749)

AWBK32749 10 reactions
EUR 647
Description:
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OLIG2 Western Blot kit (AWBK32753)

AWBK32753 10 reactions
EUR 647
Description:
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ENG Western Blot kit (AWBK33068)

AWBK33068 10 reactions
EUR 647
Description:
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SOX5 Western Blot kit (AWBK33323)

AWBK33323 10 reactions
EUR 647
Description:
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SOX10 Western Blot kit (AWBK33326)

AWBK33326 10 reactions
EUR 647
Description:
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RPS16 Western Blot kit (AWBK33535)

AWBK33535 10 reactions
EUR 647
Description:
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ATP7A Western Blot kit (AWBK33797)

AWBK33797 10 reactions
EUR 647
Description:
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A1BG Western Blot kit (AWBK33810)

AWBK33810 10 reactions
EUR 647
Description:
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  • Species reactivity: Human
  • Application:
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NPM1 Western Blot kit (AWBK34094)

AWBK34094 10 reactions
EUR 647
Description:
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LIG4 Western Blot kit (AWBK34122)

AWBK34122 10 reactions
EUR 647
Description:
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RNF2 Western Blot kit (AWBK34290)

AWBK34290 10 reactions
EUR 647
Description:
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ZFP36 Western Blot kit (AWBK34385)

AWBK34385 10 reactions
EUR 647
Description:
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ARIH2 Western Blot kit (AWBK34418)

AWBK34418 10 reactions
EUR 647
Description:
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KEAP1 Western Blot kit (AWBK34728)

AWBK34728 10 reactions
EUR 647
Description:
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CLIC1 Western Blot kit (AWBK35045)

AWBK35045 10 reactions
EUR 647
Description:
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KCNN2 Western Blot kit (AWBK35094)

AWBK35094 10 reactions
EUR 647
Description:
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CLCN3 Western Blot kit (AWBK35504)

AWBK35504 10 reactions
EUR 647
Description:
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P2RX7 Western Blot kit (AWBK35518)

AWBK35518 10 reactions
EUR 647
Description:
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TRPM3 Western Blot kit (AWBK35609)

AWBK35609 10 reactions
EUR 647
Description:
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PITX2 Western Blot kit (AWBK35634)

AWBK35634 10 reactions
EUR 647
Description:
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ILF2 Western Blot kit (AWBK35731)

AWBK35731 10 reactions
EUR 647
Description:
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  • Species reactivity:
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ONECUT2 Western Blot kit (AWBK35750)

AWBK35750 10 reactions
EUR 647
Description:
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  • Species reactivity:
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ZFP42 Western Blot kit (AWBK36244)

AWBK36244 10 reactions
EUR 647
Description:
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  • Species reactivity:
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ANXA1 Western Blot kit (AWBK36568)

AWBK36568 10 reactions
EUR 647
Description:
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  • Species reactivity:
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SPP1 Western Blot kit (AWBK36677)

AWBK36677 10 reactions
EUR 647
Description:
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  • Species reactivity:
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RUNX2 Western Blot kit (AWBK36678)

AWBK36678 10 reactions
EUR 647
Description:
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  • Species reactivity:
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ZBP1 Western Blot kit (AWBK36711)

AWBK36711 10 reactions
EUR 647
Description:
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  • Species reactivity:
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Bapx1 Western Blot kit (AWBK36826)

AWBK36826 10 reactions
EUR 647
Description:
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  • Species reactivity:
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G3BP Western Blot kit (AWBK37713)

AWBK37713 10 reactions
EUR 647
Description:
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TWIST1 Western Blot kit (AWBK37997)

AWBK37997 10 reactions
EUR 647
Description:
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  • Species reactivity:
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ID4 Western Blot kit (AWBK38058)

AWBK38058 10 reactions
EUR 647
Description:
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  • Species reactivity:
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SATB1 Western Blot kit (AWBK38208)

AWBK38208 10 reactions
EUR 647
Description:
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EED Western Blot kit (AWBK38384)

AWBK38384 10 reactions
EUR 647
Description:
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  • Species reactivity:
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TARDBP Western Blot kit (AWBK38941)

AWBK38941 10 reactions
EUR 647
Description:
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  • Species reactivity:
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TARDBP Western Blot kit (AWBK38942)

AWBK38942 10 reactions
EUR 647
Description:
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  • Species reactivity:
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BRD7 Western Blot kit (AWBK39018)

AWBK39018 10 reactions
EUR 647
Description:
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  • Species reactivity:
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JMJD3 Western Blot kit (AWBK40102)

AWBK40102 10 reactions
EUR 647
Description:
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  • Species reactivity:
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SNRP70 Western Blot kit (AWBK40439)

AWBK40439 10 reactions
EUR 647
Description:
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  • Species reactivity:
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SSB Western Blot kit (AWBK40462)

AWBK40462 10 reactions
EUR 647
Description:
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  • Species reactivity:
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STAU1 Western Blot kit (AWBK40537)

AWBK40537 10 reactions
EUR 647
Description:
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PCBP1 Western Blot kit (AWBK40630)

AWBK40630 10 reactions
EUR 647
Description:
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HNRPLL Western Blot kit (AWBK41102)

AWBK41102 10 reactions
EUR 647
Description:
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MSI2 Western Blot kit (AWBK41112)

AWBK41112 10 reactions
EUR 647
Description:
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HNRPA3 Western Blot kit (AWBK41195)

AWBK41195 10 reactions
EUR 647
Description:
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WNT2B Western Blot kit (AWBK41254)

AWBK41254 10 reactions
EUR 647
Description:
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GNB1L Western Blot kit (AWBK41328)

AWBK41328 10 reactions
EUR 647
Description:
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SLC12A1 Western Blot kit (AWBK41388)

AWBK41388 10 reactions
EUR 647
Description:
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TPM1 Western Blot kit (AWBK41392)

AWBK41392 10 reactions
EUR 647
Description:
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CEACAM6 Western Blot kit (AWBK41504)

AWBK41504 10 reactions
EUR 647
Description:
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SLC22A1 Western Blot kit (AWBK41516)

AWBK41516 10 reactions
EUR 647
Description:
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  • Species reactivity:
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STK11 Western Blot kit (AWBK41743)

AWBK41743 10 reactions
EUR 647
Description:
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OTC Western Blot kit (AWBK41766)

AWBK41766 10 reactions
EUR 647
Description:
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TSHR Western Blot kit (AWBK41870)

AWBK41870 10 reactions
EUR 647
Description:
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RHOBTB1 Western Blot kit (AWBK41883)

AWBK41883 10 reactions
EUR 647
Description:
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KRT13 Western Blot kit (AWBK42031)

AWBK42031 10 reactions
EUR 647
Description:
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IDH3A Western Blot kit (AWBK42237)

AWBK42237 10 reactions
EUR 647
Description:
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SLC1A5 Western Blot kit (AWBK42247)

AWBK42247 10 reactions
EUR 647
Description:
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PBEF1 Western Blot kit (AWBK42255)

AWBK42255 10 reactions
EUR 647
Description:
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FST Western Blot kit (AWBK42287)

AWBK42287 10 reactions
EUR 647
Description:
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SLC22A7 Western Blot kit (AWBK42306)

AWBK42306 10 reactions
EUR 647
Description:
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ASPN Western Blot kit (AWBK42487)

AWBK42487 10 reactions
EUR 647
Description:
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RSAD2 Western Blot kit (AWBK42696)

AWBK42696 10 reactions
EUR 647
Description:
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PANX2 Western Blot kit (AWBK42778)

AWBK42778 10 reactions
EUR 647
Description:
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PANX1 Western Blot kit (AWBK42783)

AWBK42783 10 reactions
EUR 647
Description:
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TGFBI Western Blot kit (AWBK44268)

AWBK44268 10 reactions
EUR 647
Description:
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PTPN2 Western Blot kit (AWBK45362)

AWBK45362 10 reactions
EUR 647
Description:
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RORA Western Blot kit (AWBK45608)

AWBK45608 10 reactions
EUR 647
Description:
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Chemiluminescent Western Blot Kit (OKRA00001)

OKRA00001 1 kit
EUR 570
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00002)

OKRA00002 1 kit
EUR 570
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00003)

OKRA00003 1 kit
EUR 570
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Chemiluminescent Western Blot Kit (OKRA00004)

OKRA00004 1 kit
EUR 570
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00014)

OKRA00014 1 kit
EUR 609
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00015)

OKRA00015 1 each
EUR 609
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00016)

OKRA00016 1 kit
EUR 609
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Colorimetric Western Blot Kit (OKRA00017)

OKRA00017 1 kit
EUR 609
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

T-Pro Western Blot Stripping Reagent

JB11-K002 500ml/BT
EUR 178

Western Blot Primary Antibody Diluent Buffer

abx090689-100ml 100 ml
EUR 182
  • Shipped within 5-10 working days.

ChonBlock Western Blot Buffer 1 (PBS)

9069P 10 ml
EUR 193.55
Description: ChonBlock Western Blot Buffer 1 (PBS)

ChonBlock Western Blot Buffer 1 (TBS)

9069T 10 ml
EUR 193.55
Description: ChonBlock Western Blot Buffer 1 (TBS)

Pure-IP Western Blot Detection Kit

PRB-5002 20 blots
EUR 392
Description: Western blot detection of IP proteins can exhibit high background due to the use of the same primary antibody in the IP and the Western blot. The Pure-IP Western Blot Detection Kit eliminates this background problem by using a proprietary HRP Conjugate for detection of the primary antibody.

cyclin G2 Western Blot kit (AWBK03032)

AWBK03032 10 reactions
EUR 647
Description:
  • Description of target:
  • Species reactivity:
  • Application:
  • Assay info:
  • Sensitivity:

Poly-ADP-ribosylated proteins for Western blot

ADPR11-C 100 ul
EUR 286

Mouse Ceruloplasmin protein control for western blot

CP16-C 100 ul
EUR 286

Rat Ceruloplasmin protein control for western blot

CP17-C 100 ul
EUR 286

Recombinant Human MutY Protein for Western blot

MUTY11-C 100 ul
EUR 286

Nitrated Mol Wt Std for Western blot

NITT12-C 100 ul
EUR 286

SuperBrite? ECL Western Blot Substrate/Detection Kit

K824-50
EUR 191

Chicken Serum Albumin protein control for Western blot

ALBC15-C 100 ul
EUR 286

Beta-Galactosidase (E. Coli) Protein for Western Blot

BGAL11-C 100 ul
EUR 286

Recombinant Beta Lactamase protein control for Western blot

BLAC11-C 100 ul
EUR 286

TK-2, some chilly tolerance genes have been detected, which implied the distinctive adaptability of Bacillus sp. TK-2 in long-term low temperature environments. Importantly, enzyme-encoding genes associated to the degradation of polysaccharides such as cellulose and hemicellulose have been detected within the 477 CAZyme genes of this genome. This work on sequencing and bioinformatics evaluation of the entire sequence of Bacillus sp. TK-2 promote the appliance and in-depth analysis of low-temperature biotechnology.